Hi everyone!

Let me start off by saying, I am a chemist so I'm really butchering the biology side of things. Follow along though. I have bacterial extracts, in 1-1.5mL eppendorf tubes, but I am trying to grow up large scale extracts for chemical analysis. I'm starting with 2mL broth solutions and I'm trying to go to 1L. Once I make my glycerol stocks, I'm thinking of going up to 1L in this manner: 75uL in 15mL -> 15mL in 250mL -> 250mL in 1L. So the 75uL, 15mL, and 250mL would serve as my "seed" broths, if I explained that correctly. My end goal is to partition with ethyl acetate and look at the metabolites, so would my options be to either centrifuge, filter, or partition the 1L broth? Again, this is where the microbiology/biology goes fuzzy for me. I'd appreciate any help though!