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HELP!! How long can fixed tissue (mouse retina/optic nerve) be kept in 30% sucrose?
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Hey all! Preparing some retina/optic nerve tissue for cryosectioning this weekend. They’ve been fixed in 4% PFA, then soaked step-wise in 15% and 30% sucrose. I’m alone in lab this weekend, and offff course the whole building is out of liquid nitrogen AND dry ice. Do you all know of any alternative storage methods for my samples over the weekend till we get more extra cold stuff? Or can I potentially leave them in the 30% sucrose solution?
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