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If a protocol asks for 1% BSA to be supplemented to a buffer, should it be added fresh?
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As the title says, I have a protocol asking for a isolating buffer supplemented with 1X PIC and 1% BSA. I know Iām supposed to add the PIC fresh every time, but should the BSA also be added fresh? Or can I make the buffer ahead of time with the BSA and just add PIC fresh?
Clarification: the buffer is just KCL, MgCl2, KH2PO4, HEPES, edta, and the supplemented PIC AND BSA
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- 4 years ago
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