Doubt on mutant protein expression

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Hey everyone,

I'm new to protein characterization and have a question that's probably quite basic but nonetheless I haven't found clear answers looking in papers/google

I want to express a catalytically inactive protein mutant to assess downstream targets. First I thought I could just overexpress the mutant using a viral vector, but then I will still have the wild-type endogenous protein with normal activity. Do people in general knockout the endogenous protein before expressing mutant proteins? What's the norm?

Thanks in advance

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Posted: 10 months ago
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