I am referring to these columns. I am producing a monoclonal antibody from a hybridoma cell line, and will have about 200 ml of supernatant to concentrate using these columns. The product manual says that they are single-use only, but of course that's what they would say to get you to buy more columns.

Does anyone have experience with these or similar columns, and could say whether they are re-usable? I am collecting about 200 ml of supernatant over the next couple of days, and want to concentrate all of it in one session. If I use 4 of these columns (which take 15 ml per spin), and re-use them about 3 times in immediate succession (i.e. the membranes will not dry in between uses), that would take care of all my supernatant and get the job done fairly cheaply. Do you think my recovery will be affected by re-using?