Hi all,

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I'm very new to informatics and my PI, who is also new, is keen that I analyse my RNAseq data by running it thorugh EnRichR, but I'm a bit confused by the process. Firstly, EnRichR doesn't give you the option to upload a background gene list, do you not need a background gene list in order to say whether a gene set is enriched or not?

Secondly, my PI has suggested that I upload a list of significantly (p<0.05) differentially expressed genes (WT vs KO), and EnrichR does not require a list of ranked genes or fold change data like GSEA does, so will the up and downregulated genes not mask each other? would it not be better to submit separate lists for upregulated and downregulated genes?

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Thanks in advance