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[Technique] Liquid culture jar questions
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TheFrostyjayjay is in Technique
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I am planning on making up a few jar lids and was wondering how long I need to let the RTV sit/cure before I can use the jars?

Also, do you need a gas exchange hole? I have seen information leaning both ways. Some say it isn't needed at all, and others do.

What is the point of an airport hole made with a syringe? Seems over the top. Just to keep the pressure from messing you up?

If I do need some sort of gas exchange, can't I just make a hole and cover it with micropore tape? Which would also solve the issue with pressure, which also seems simpler than an airport syringe.

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4 years ago