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Made from http://chopchop.cbu.uib.no
https://chopchop.cbu.uib.no/results/1583089233820.9504/
https://pastebin.com/tgFMTrzJ
"Knock-out"
Rank Target sequence Genomic location Strand GC content (%) Self-complementarity Efficiency MM0 MM1 MM2 MM3
1 TTCCATTTGTAGTTTCAACTGGATACCA seq:1013 33 0 0 1 0 0 0
2 TTCAACTGGATACCACTTCAGAGAGCTA seq:1026 46 0 0 1 0 0 0
3 TTCAGAGAGCTAGGTGTTGTACATAATC seq:1042 42 0 0 1 0 0 0
4 TTCCTTAAAACTAAGTCTAGAGCTATGT seq:1083 - 29 0 0 1 0 0 0
5 TTCTGGTAATCTATTACTAGATAAACGC seq:1149 33 0 0 1 0 0 0
6 TTCAGTAGCTGCACTTACTAACAATGTT seq:1188 38 0 0 1 0 0 0
7 TTCAAACTGTCAAACCCGGTAATTTTAA seq:1220 33 0 0 1 0 0 0
8 TTCTATGACTTTGCTGTGTCTAAGGGTT seq:1255 42 0 0 1 0 0 0
9 TTCCTTAAAGAAACCCTTAGACACAGCA seq:1266 - 38 0 0 1 0 0 0
10 TTCCTTCCTTAAAGAAACCCTTAGACAC seq:1270 - 38 0 0 1 0 0 0
11 TTCAACAGAACTTCCTTCCTTAAAGAAA seq:1281 - 33 0 0 1 0 0 0
12 TTCTTTAAGGAAGGAAGTTCTGTTGAAT seq:1282 33 0 0 1 0 0 0
13 TTCTGTTGAATTAAAACACTTCTTCTTT seq:1299 25 0 0 1 0 0 0
14 TTCTTCTTTGCTCAGGATGGTAATGCTG seq:1318 46 0 0 1 0 0 0
15 TTCTTTGCTCAGGATGGTAATGCTGCTA seq:1321 46 0 0 1 0 0 0
16 TTCCTAAAAACTAATTGTTGTCGCTTCC seq:142 33 0 0 1 0 0 0
17 TTCTTGGAAGCGACAACAATTAGTTTTT seq:147 - 33 0 0 1 0 0 0
18 TTCCATTTAATAAATGGGGTAAGGCTAG seq:1511 33 0 0 1 0 0 0
19 TTCGTCCTTTTCTTGGAAGCGACAACAA seq:156 - 42 0 0 1 0 0 0
20 TTCAATGAGTTATGAGGATCAAGATGCA seq:1551 38 0 0 1 0 0 0
21 TTCGCATATACAAAACGTAATGTCATCC seq:1582 33 0 0 1 0 0 0
22 TTCATTTGAGTTATAGTAGGGATGACAT seq:1601 - 33 0 0 1 0 0 0
23 TTCTTTGCACTAATGGCATACTTAAGAT seq:1628 - 33 0 0 1 0 0 0
24 TTCCAAGAAAAGGACGAAGATGACAATT seq:166 33 0 0 1 0 0 0
25 TTCAATAATTTTTGATGAAACTGTCTAT seq:1703 - 21 0 0 1 0 0 0
26 TTCATCAAAAATTATTGAAATCAATAGC seq:1712 21 0 0 1 0 0 0
27 TTCTACATCACTATAAACAGTTTTTAAC seq:1803 - 25 0 0 1 0 0 0
28 TTCTATAGATTAGCTAATGAGTGTGCTC seq:1954 38 0 0 1 0 0 0
29 TTCTTACTTTGTAGTTAAGAGACACACT seq:201 33 0 0 1 0 0 0
30 TTCCACCTGGTTTAACATATAGTGAACC seq:2011 - 38 0 0 1 0 0 0
31 TTCACTATATGTTAAACCAGGTGGAACC seq:2013 42 0 0 1 0 0 0
32 TTCTATAGAGACACTCATAAAGTCTGTG seq:2173 - 38 0 0 1 0 0 0
33 TTCATGTTGGTAGTTAGAGAAAGTGTGT seq:222 - 38 0 0 1 0 0 0
34 TTCTTCATGTTGGTAGTTAGAGAAAGTG seq:225 - 38 0 0 1 0 0 0
35 TTCTCAATGATGATACTCTCTGACGATG seq:2257 42 0 0 1 0 0 0
36 TTCTCTAACTACCAACATGAAGAAACAA seq:229 33 0 0 1 0 0 0
37 TTCAATAGCACTTATGCATCTCAAGGTC seq:2296 42 0 0 1 0 0 0
38 TTCTTTATTATCAAAACAATGTTTTTAT seq:2354 12 0 0 1 0 0 0
39 TTCAGACATAAAAACATTGTTTTGATAA seq:2361 - 21 0 0 1 0 0 0
40 TTCTTGATGGATCTGGGTAAGGAAGGTA seq:2482 - 46 0 0 1 0 0 0
41 TTCCTTACCCAGATCCATCAAGAATCCT seq:2486 42 0 0 1 0 0 0
42 TTCAATCATAAGTGTACCATCTGTTTTT seq:2544 - 29 0 0 1 0 0 0
43 TTCGTGTCTTTAGCTATAGATGCTTACC seq:2575 38 0 0 1 0 0 0
44 TTCTTATGTATTGTAAGTACAAATGAAA seq:2641 - 21 0 0 1 0 0 0
45 TTCATTTGTACTTACAATACATAAGAAA seq:2642 21 0 0 1 0 0 0
46 TTCTGTTATGCTTACTAATGATAACACT seq:2709 29 0 0 1 0 0 0
47 TTCCCAATACCTTGAAGTGTTATCATTA seq:2724 - 29 0 0 1 0 0 0
48 TTCTAAACTTAAAGAAGTCATGTTTAGC seq:289 - 29 0 0 1 0 0 0
49 TTCTTTAAGTTTAGAATAGACGGTGACA seq:301 33 0 0 1 0 0 0
50 TTCAATAAAAAGGACTGGTATGATTTTG seq:469 29 0 0 1 0 0 0
51 TTCTACAAAATCATACCAGTCCTTTTTA seq:474 - 29 0 0 1 0 0 0
52 TTCGCATGGCATCACAGAATTGTACTGT seq:565 - 42 0 0 1 0 0 0
53 TTCTGTGATGCCATGCGAAATGCTGGTA seq:574 50 0 0 1 0 0 0
54 TTCGGTGATTTCATACAAACCACGCCAG seq:655 46 0 0 1 0 0 0
55 TTCCTGTTGTAGATTCTTATTATTCATT seq:692 21 0 0 1 0 0 0
56 TTCTTATTATTCATTGTTAATGCCTATA seq:705 21 0 0 1 0 0 0
57 TTCATTGTTAATGCCTATATTAACCTTG seq:714 29 0 0 1 0 0 0
58 TTCCGTGAAGTCATATTTTAACAAATCC seq:804 - 29 0 0 1 0 0 0
59 TTCTGCATTGTGCAAACTTTAATGTTTT seq:920 29 0 0 1 0 0 0
60 TTCTCTACAGTGTTCCCACCTACAAGTT seq:949 46 0 0 1 0 0 0
61 TTCCCACCTACAAGTTTTGGACCACTAG seq:961 46 0 0 1 0 0 0
62 TTCTCACTAGTGGTCCAAAACTTGTAGG seq:967 - 46 0 0 1 0 0 0
63 TTCCAATTACTACAGTAGCTCCTCTAGT seq:1744 - 38 1 0 1 0 0 0
64 TTCTATGGTGGTTGGCACAACATGTTAA seq:1780 42 1 0 1 0 0 0
65 TTCTAAGCATGTTAGGCATGGCTCTATC seq:1867 - 46 1 0 1 0 0 0
66 TTCTTGCTCGCAAACATACAACGTGTTG seq:1910 46 1 0 1 0 0 0
67 TTCACTCAATACTTGAGCACACTCATTA seq:1968 - 38 1 0 1 0 0 0
68 TTCACAAAGTCTGTGTCAACATCTCTAT seq:2201 - 38 1 0 1 0 0 0
69 TTCTTTATGCTAGCCACTAGACCTTGAG seq:2315 - 46 1 0 1 0 0 0
70 TTCATGAGGTCCTTTAGTAAGGTCAGTC seq:2406 - 46 1 0 1 0 0 0
71 TTCAAGGTATTGGGAACCTGAGTTTTAT seq:2736 38 1 0 1 0 0 0
72 TTCATCAAAATGCCTTAAAGCATAGACG seq:381 - 38 1 0 1 0 0 0
73 TTCTTTTAATGTGTCACAATTACCTTCA seq:405 - 29 1 0 1 0 0 0
74 TTCACCTAAGTTGGCGTATACGCGTAAT seq:513 - 46 1 0 1 0 0 0
75 TTCATACAAACCACGCCAGGTAGTGGAG seq:664 54 1 0 1 0 0 0
76 TTCAACTACAAATAGTAGTTGTCTGATA seq:1395 - 29 2 0 1 0 0 0
77 TTCACGGAAGAGAGGTTAAAACTCTTTG seq:823 42 2 0 1 0 0 0
Polyamerase RNA
gtcagctgatgcacaatcgtttttaaacgggtttgcggtgtaagtgcagcccgtcttacaccgtgcggcacaggcactagtactgatgtcgtatacagggcttttgacatctacaatgataaagtagctggttttgctaaattcctaaaaactaattgttgtcgcttccaagaaaaggacgaagatgacaatttaattgattcttactttgtagttaagagacacactttctctaactaccaacatgaagaaacaatttataatttacttaaggattgtccagctgttgctaaacatgacttctttaagtttagaatagacggtgacatggtaccacatatatcacgtcaacgtcttactaaatacacaatggcagacctcgtctatgctttaaggcattttgatgaaggtaattgtgacacattaaaagaaatacttgtcacatacaattgttgtgatgatgattatttcaataaaaaggactggtatgattttgtagaaaacccagatatattacgcgtatacgccaacttaggtgaacgtgtacgccaagctttgttaaaaacagtacaattctgtgatgccatgcgaaatgctggtattgttggtgtactgacattagataatcaagatctcaatggtaactggtatgatttcggtgatttcatacaaaccacgccaggtagtggagttcctgttgtagattcttattattcattgttaatgcctatattaaccttgaccagggctttaactgcagagtcacatgttgacactgacttaacaaagccttacattaagtgggatttgttaaaatatgacttcacggaagagaggttaaaactctttgaccgttattttaaatattgggatcagacataccacccaaattgtgttaactgtttggatgacagatgcattctgcattgtgcaaactttaatgttttattctctacagtgttcccacctacaagttttggaccactagtgagaaaaatatttgttgatggtgttccatttgtagtttcaactggataccacttcagagagctaggtgttgtacataatcaggatgtaaacttacatagctctagacttagttttaaggaattacttgtgtatgctgctgaccctgctatgcacgctgcttctggtaatctattactagataaacgcactacgtgcttttcagtagctgcacttactaacaatgttgcttttcaaactgtcaaacccggtaattttaacaaagacttctatgactttgctgtgtctaagggtttctttaaggaaggaagttctgttgaattaaaacacttcttctttgctcaggatggtaatgctgctatcagcgattatgactactatcgttataatctaccaacaatgtgtgatatcagacaactactatttgtagttgaagttgttgataagtactttgattgttacgatggtggctgtattaatgctaaccaagtcatcgtcaacaacctagacaaatcagctggttttccatttaataaatggggtaaggctagactttattatgattcaatgagttatgaggatcaagatgcacttttcgcatatacaaaacgtaatgtcatccctactataactcaaatgaatcttaagtatgccattagtgcaaagaatagagctcgcaccgtagctggtgtctctatctgtagtactatgaccaatagacagtttcatcaaaaattattgaaatcaatagccgccactagaggagctactgtagtaattggaacaagcaaattctatggtggttggcacaacatgttaaaaactgtttatagtgatgtagaaaaccctcaccttatgggttgggattatcctaaatgtgatagagccatgcctaacatgcttagaattatggcctcacttgttcttgctcgcaaacatacaacgtgttgtagcttgtcacaccgtttctatagattagctaatgagtgtgctcaagtattgagtgaaatggtcatgtgtggcggttcactatatgttaaaccaggtggaacctcatcaggagatgccacaactgcttatgctaatagtgtttttaacatttgtcaagctgtcacggccaatgttaatgcacttttatctactgatggtaacaaaattgccgataagtatgtccgcaatttacaacacagactttatgagtgtctctatagaaatagagatgttgacacagactttgtgaatgagttttacgcatatttgcgtaaacatttctcaatgatgatactctctgacgatgctgttgtgtgtttcaatagcacttatgcatctcaaggtctagtggctagcataaagaactttaagtcagttctttattatcaaaacaatgtttttatgtctgaagcaaaatgttggactgagactgaccttactaaaggacctcatgaattttgctctcaacatacaatgctagttaaacagggtgatgattatgtgtaccttccttacccagatccatcaagaatcctaggggccggctgttttgtagatgatatcgtaaaaacagatggtacacttatgattgaacggttcgtgtctttagctatagatgcttacccacttactaaacatcctaatcaggagtatgctgatgtctttcatttgtacttacaatacataagaaagctacatgatgagttaacaggacacatgttagacatgtattctgttatgcttactaatgataacacttcaaggtattgggaacctgagttttatgaggctatgtacacaccgcatacagtcttacagg
EXPASY translation tool: https://web.expasy.org/translate/
Full Coronavirus genome: https://www.ncbi.nlm.nih.gov/nuccore/MN908947.3?report=fasta
RNA polyamerase https://www.ncbi.nlm.nih.gov/protein/YP_009725307.1?report=genpept
Coronavirus 3'5' exoribonuclease
gctgaaaatgtaacaggactctttaaagattgtagtaaggtaatcactgggttacatcctacacaggcacctacacacctcagtgttgacactaaattcaaaactgaaggtttatgtgttgacatacctggcatacctaaggacatgacctatagaagactcatctctatgatgggttttaaaatgaattatcaagttaatggttaccctaacatgtttatcacccgcgaagaagctataagacatgtacgtgcatggattggcttcgatgtcgaggggtgtcatgctactagagaagctgttggtaccaatttacctttacagctaggtttttctacaggtgttaacctagttgctgtacctacaggttatgttgatacacctaataatacagatttttccagagttagtgctaaaccaccgcctggagatcaatttaaacacctcataccacttatgtacaaaggacttccttggaatgtagtgcgtataaagattgtacaaatgttaagtgacacacttaaaaatctctctgacagagtcgtatttgtcttatgggcacatggctttgagttgacatctatgaagtattttgtgaaaataggacctgagcgcacctgttgtctatgtgatagacgtgccacatgcttttccactgcttcagacacttatgcctgttggcatcattctattggatttgattacgtctataatccgtttatgattgatgttcaacaatggggttttacaggtaacctacaaagcaaccatgatctgtattgtcaagtccatggtaatgcacatgtagctagttgtgatgcaatcatgactaggtgtctagctgtccacgagtgctttgttaagcgtgttgactggactattgaatatcctataattggtgatgaactgaagattaatgcggcttgtagaaaggttcaacacatggttgttaaagctgcattattagcagacaaattcccagttcttcacgacattggtaaccctaaagctattaagtgtgtacctcaagctgatgtagaatggaagttctatgatgcacagccttgtagtgacaaagcttataaaatagaagaattattctattcttatgccacacattctgacaaattcacagatggtgtatgcctattttggaattgcaatgtcgatagatatcctgctaattccattgtttgtagatttgacactagagtgctatctaaccttaacttgcctggttgtgatggtggcagtttgtatgtaaataaacatgcattccacacaccagcttttgataaaagtgcttttgttaatttaaaacaattaccatttttctattactctgacagtccatgtgagtctcatggaaaacaagtagtgtcagatatagattatgtaccactaaagtctgctacgtgtataacacgttgcaatttaggtggtgctgtctgtagacatcatgctaatgagtacagattgtatctcgatgcttataacatgatgatctcagctggctttagcttgtgggtttacaaacaatttgatacttataacctctggaacacttttacaagacttcaga
Programmable Inhibition and Detection of RNA Viruses Using Cas13 https://www.cell.com/molecular-cell/fulltext/S1097-2765(19)30698-7
crRNA design helper
http://bioinfolab.miamioh.edu/CRISPR-RT/interface/C2c2.php
AsCpf1 guided nuclease on-target genomic hits of gRNA for coronavirus polyamerase
FnCpf1 guided nuclease on-target genomic hits of gRNA for coronavirus polyamerase
LbCpf1 guided nuclease on-target genomic hits of gRNA for coronavirus polyamerase
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