This post has been de-listed
It is no longer included in search results and normal feeds (front page, hot posts, subreddit posts, etc). It remains visible only via the author's post history.
9
[Spectrophotometry] [Turbidimetry] Questioning our lab protocol in turbidimetry: Shouldn't sterile broth be the more appropriate blank?
Post Flair (click to view more posts with a particular flair)
Author Summary
backupalter1 is
in
Turbidimetry
Post Body
Also posted on r/biology and r/microbiology
Part of our protocol is having the original bacterial suspension diluted into suspension:diluent solutions, namely 1:2, 1:3, 1:4, and 1:5. The diluent is sterile 0.85% saline solution. When it's time to use the spectrophotometer, the suggested blank is sterile 0.85% saline solution. Isn't the sterile broth a more appropriate blank? Maybe add saline to it to 0.85%?
Author
Account Strength
80%
Account Age
2 years
Verified Email
Yes
Verified Flair
No
Total Karma
5,900
Link Karma
3,750
Comment Karma
2,077
Profile updated: 7 months ago
Posts updated: 5 hours ago
Subreddit
Post Details
Location
We try to extract some basic information from the post title. This is not
always successful or accurate, please use your best judgement and compare
these values to the post title and body for confirmation.
- Posted
- 2 years ago
- Reddit URL
- View post on reddit.com
- External URL
- reddit.com/r/Biochemistr...