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Purification of short length PCR products 80 bp
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Hi guys, I'm a PhD student currently working on developing a SELEX method, I need to purify my ssDNA (separated from ds PCR product via affinity chromatography) prior to each round of SELEX to eliminate bias in the selection stage, however, looking at PCR cleanup kits and other methods for doing this I haven't found any that are optimised for DNA below 100 bp in length, my oligos are 80 bp.

Could someone point me in the right direction for a protocol optimised for this?

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3 years ago