Tips for preparing HPLC standards from crystalline?

This post has been de-listed

It is no longer included in search results and normal feeds (front page, hot posts, subreddit posts, etc). It remains visible only via the author's post history.

Post Body

I'm looking to create a cocktail of fat-soluble vitamins to run on our RP-HPLC for students to have a look at as a standard to then compare isolated samples against.

I've never prepared standards before but will be looking to order the following from Sigma

Retinol (Vita A)
Ergocalciferol (Vita D)
Alpha tocopherol (Vita E)
Phytonadione (Vita K)

As most of these are fat-soluble i was hoping to use something like Acetonitrile and water as the mobile phase.

Does anyone have suggestions on how to prep standards from a powder? Would I mix them in an aliquot of the mobile phase?

Any other thoughts greatly appreciated!

Author

Account Strength

100%

Account Age

4 years

Verified Email

Yes

Verified Flair

Total Karma

3,411

Link Karma

2,461

Comment Karma

401

Profile updated: 4 days ago

Posts updated: 5 months ago

SciencePeddler

Subreddit

r/Biochemistry

Post Details

We try to extract some basic information from the post title. This is not always successful or accurate, please use your best judgement and compare these values to the post title and body for confirmation.

Posted: 3 years ago
Reddit URL: View post on reddit.com
External URL: reddit.com/r/Biochemistr...