Hey guys, it’s my first time posting on here so go easy lol

I have an assignment where we have to relate specific structural changes in a protein and how those alter the functionality of the protein in question. This is a completely open ended assignment.

I chose the Erythropoietin Receptor bound to Erythropoietin.

I was thinking of altering the amino acid sequence of the protein and changing some parts of the protein in question from an alpha helix to a beta sheet and maybe altering a hydrophobic membrane bound domain to a hydrophilic one to see how that would alter the overall function but I wanted to tie in more concepts.

So my question is, what other structural aspects could I change of my protein using Deepview that would change the alter the function of the protein? It doesn’t matter if it becomes more efficiently functional or non-functional all together as long as I justify the changes I am making

Some proposed hypothesis my professor has recommended are listed below:

1.Hypothesize the effect on function of changes in primary amino acid sequence, in the context of disease or in the process of developing novel functions.

1. Modeling of the potential tertiary structure of a protein based on the known structure of similar proteins as for example modeling the structure of an alternative splice variant protein based on the structure of the wild type protein.

2. Analysis of the function of conserved amino acids in a family of homologous proteins.

Any suggestions you guys have will be appreciated!