Anyone with some experience in Isothermal Titration Calorimetry able to offer advice?

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Hi all,

I'm running some ITC experiments on some aptamers against a target protein.

I'm having to train myself on running the instrument by YouTube videos/papers/trial and error as noone in the department knows how to use it (I'm a PhD student)

I've got one control (EDTA CaCl2 titration) running fine.

However, in the case of my second positive control, titration of 10 uM BSA with 100 uM of a BSA aptamer things look a bit weird

The peaks are very broad compared to everything I see in literature (thrombin/VEGF aptamer binding etc)

I'm at the max rpm for the instrument, I'm unsure what else could be causing this issue

If you could offer any help or point me in the direction of some resources to better understand this technique I'd be really grateful!

Let me know if you need more information

Cheers

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edwardc140595

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