454 sequencing - PCR product (800 bp) too long?

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454 sequencing - PCR product (800 bp) too long?

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zephirum is age 45

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Hi all,

Recently our lab is trying to sequence some environmental archaeal 16S DNA through 454. The sequencing failed and we were told (by the sequencing people who never dealt with archaea before) that our PCR products were too long (~800 bp).

My question is: Would a too long product completely fail the sequencing process? (I suppose also the library preparation step as well). My experience only extend to Sanger sequencing where the read quality drops off past the optimal range, would it be the same for 454 or would the read fail completely?

Thanks in advance!

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zephirum

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Posted: 11 years ago
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